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SETBP1 in Myeloid Malignancies—Putting Breadcrumbs Together in Rare, Uncommon, and Common Pediatric Cases
CC BY 4.0 · Indian J Med Paediatr Oncol 2024; 45(01): 092-094
DOI: DOI: 10.1055/s-0043-1769491
In resource-constraint settings, genetic testing is rarely available for patients. We report the association of SETBP1, a major oncogene of interest in myeloid malignancies, with juvenile myelomonocytic leukemia (JMML) and with GATA 1 mutation. The modulating role of SETBP1 in Rat sarcoma virus (RAS)-driven myeloid malignancies, especially JMML, is unknown. In vivo and in vitro studies point to the role of SETBP1 in aggressive leukemogenesis.[1] SETBP1 mutation is not considered the primary event.[2] The association of SETBP1 with RAS and PTPN11, protein tyrosine phosphatase nonreceptor type 11 (PTPN11) confers both self-renewal and oncogenic transformation. The outcome of SETBP1 with confounding mutations like PTPN11 and interlinked pathways of Mitogen-activated protein kinase (MAPK) may be difficult to predict.[3] [4] PTPN11 in co-association with RAS in JMML is an independent poor prognostic factor[5] and confounds the effects of those associated with SETBP1. PTPN11 also has poor prognostic outcomes post-hematopoietic stem cell transplantation in JMML patients.[3]
We present three cases of rare associations of SETBP1 with GATA1 mutation, JMML, and one with concomitant PTPN11 mutation. Our first case was a novel association of SETBP1 in transient abnormal myelopoiesis (TAM) with a fatal outcome, the second case was JMML with a blastic transformation-like presentation with tumor lysis, and the third case was a case of JMML. These different outcomes could be attributed to the association of an underlying SETBP1 or one of the pathway mutations downstream of myeloid leukemogenesis. The presentation, however, in all cases was similar: severe anemia, massive organomegaly, presentation with high leucocyte count, and tumor lysis syndrome in one patient (another rare occurrence in JMML) ([Table 1]). Two of the patients died within 4 days despite the best supportive treatment. One of the JMML cases with isolated SETBP1 mutation has an indolent course compared to the other two and is alive on follow-up awaiting transplantation ([Table 1]). SETBP1 may not confer a dismal outcome and is just evidence of complex mutagenesis and could also mean heterogeneous clones with isolated mutations rather than co-existent mutations.
|
Case |
1. TAM with SETBP1 case |
2. Uncommon SETBP1 mutated JMML case |
3. Common SETBP1 in JMML |
|---|---|---|---|
|
Brief history |
A 2-day-old baby boy born out of nonconsanguineous marriage, 3rd in birth order presented with leucocytosis, thrombocytopenia and anemia with myeloid blasts |
A 1-year-old child presented with a history of progressive paleness of the body, frequent infections, fever, and abdominal distension for 1 month. The child had monocytic leucocytosis, 20%-blasts on peripheral smear with laboratory evidence of tumor lysis syndrome |
A 1-year-old male child born out of nonconsanguineous marriage, presented with complaints of fever with abdominal distension for 3 months |
|
Primary investigations |
Flow cytometry: AML with MPO + CD34 + CD117 + CD33 + , CD56 + , and CD7dim+ ([Fig. 1]) |
Investigations suggested myeloid blast crisis in a known case of JMML with tumor lysis syndrome |
Investigations showed leucocytosis with monocytosis, thrombocytopenia, and massive hepatosplenomegaly. The child met the criteria for JMML on the investigations (Splenomegaly, 8%-blasts on Peripheral smear, leucocytosis, absolute monocytosis, myeloid precursors |
|
Sequencing result |
GATA1, SETBP1 |
PTPN11, SETBP1 |
PTPN11, SETBP1 |
|
Details of variants |
1. Delins variant (chrX:g.48791258_48791271delinsTCAGGCAGT; c.149_162delinsTCAGGCAGT) in the GATA1 gene (561X, 8.4%-MAF). 2. Frameshift deletion (chrX:g.48791243_48791246del; c.134_137del) 560X, 24.8%-MAF in GATA1 gene ([Fig. 1]) 3. SETBP1 gene mutation at c.2843G > A (ENST00000649279.2) in Exon 4 was found (859X, MAF- 48.9%) ([Fig. 1]) |
1. PTPN11 mutation {(chr12:g.112450406G > A; c.226G > A) not reported in ExAC and 1000 genomes databases[VCV000013336.5]} 2. SETBP1 mutation [(chr18:g.44951942G > A; c.2602G > A) gain-of-function mutation MAF-0.002%, not reported in 1000 genomes database] (Fig. 2A and B) |
1. PTPN11 gene [c.179G > T (ENST00000635625.1) 51X, MAF- 49.3%]. 2. SETBP1mutation [c.1102A > G (ENST00000649279.2) with heterozygous allele burden (50%) and 258X depth read (Fig. 3A and B) |
|
Treatment |
Supportive |
Supportive |
On supportive transfusion care, awaiting BMT |
|
Outcome |
Mortality at day 2 of admission |
Mortality at day 4 of admission |
Alive and hemodynamically stable on transfusion |
|
Remarks |
Never reported in literature co-association of SETBP1 with GATA1in a patient could highlight the inter-related myeloid pathway in Leukemia and MPNS |
The child was detected to have concurrent. The rare presentation as tumor lysis syndrome could be attributable to co-existent PTPN-11 and SETBP1 mutation in the same clone by simultaneous or additional hit |
SETBP1 in JMML in our case three did not have a dismal outcome, suggesting the SETBP1 and PTPN11 could be heteroclonal or subclonal, rather than concomitant in single malignant clone |
| Fig 1 :The morphological features, flow-cytometry characters, and sequencing result of patient 1 in the left-side plane and the sequencing results for patients 2 (2A and B) and patient 3 (3A and B) on the right-side pane.
SETBP1 co-expression leads to increased phosphorylated-driven MAP kinases, with a possible role of trametinib in these disorders.[2] Co-occurrence with other known mutations highlights the interrelated roles of the downstream leukemoid pathogenesis in these strikingly different neoplasms. The case of TAM with SETBP1 could initiate new insight into myeloid leukemogenesis. Next-generation sequencing is essential in the diagnostic management of all pediatric myeloproliferative disorders and can improve outcomes with better risk stratification.
Ethical Statement
All ethical consideration and permissions were obtained for preparation of manuscript.
* Joint first authors-Both contributed equally.
Publication History
Article published online:
09 June 2023
© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
In resource-constraint settings, genetic testing is rarely available for patients. We report the association of SETBP1, a major oncogene of interest in myeloid malignancies, with juvenile myelomonocytic leukemia (JMML) and with GATA 1 mutation. The modulating role of SETBP1 in Rat sarcoma virus (RAS)-driven myeloid malignancies, especially JMML, is unknown. In vivo and in vitro studies point to the role of SETBP1 in aggressive leukemogenesis.[1] SETBP1 mutation is not considered the primary event.[2] The association of SETBP1 with RAS and PTPN11, protein tyrosine phosphatase nonreceptor type 11 (PTPN11) confers both self-renewal and oncogenic transformation. The outcome of SETBP1 with confounding mutations like PTPN11 and interlinked pathways of Mitogen-activated protein kinase (MAPK) may be difficult to predict.[3] [4] PTPN11 in co-association with RAS in JMML is an independent poor prognostic factor[5] and confounds the effects of those associated with SETBP1. PTPN11 also has poor prognostic outcomes post-hematopoietic stem cell transplantation in JMML patients.[3]
We present three cases of rare associations of SETBP1 with GATA1 mutation, JMML, and one with concomitant PTPN11 mutation. Our first case was a novel association of SETBP1 in transient abnormal myelopoiesis (TAM) with a fatal outcome, the second case was JMML with a blastic transformation-like presentation with tumor lysis, and the third case was a case of JMML. These different outcomes could be attributed to the association of an underlying SETBP1 or one of the pathway mutations downstream of myeloid leukemogenesis. The presentation, however, in all cases was similar: severe anemia, massive organomegaly, presentation with high leucocyte count, and tumor lysis syndrome in one patient (another rare occurrence in JMML) ([Table 1]). Two of the patients died within 4 days despite the best supportive treatment. One of the JMML cases with isolated SETBP1 mutation has an indolent course compared to the other two and is alive on follow-up awaiting transplantation ([Table 1]). SETBP1 may not confer a dismal outcome and is just evidence of complex mutagenesis and could also mean heterogeneous clones with isolated mutations rather than co-existent mutations.
|
Case |
1. TAM with SETBP1 case |
2. Uncommon SETBP1 mutated JMML case |
3. Common SETBP1 in JMML |
|---|---|---|---|
|
Brief history |
A 2-day-old baby boy born out of nonconsanguineous marriage, 3rd in birth order presented with leucocytosis, thrombocytopenia and anemia with myeloid blasts |
A 1-year-old child presented with a history of progressive paleness of the body, frequent infections, fever, and abdominal distension for 1 month. The child had monocytic leucocytosis, 20%-blasts on peripheral smear with laboratory evidence of tumor lysis syndrome |
A 1-year-old male child born out of nonconsanguineous marriage, presented with complaints of fever with abdominal distension for 3 months |
|
Primary investigations |
Flow cytometry: AML with MPO + CD34 + CD117 + CD33 + , CD56 + , and CD7dim+ ([Fig. 1]) |
Investigations suggested myeloid blast crisis in a known case of JMML with tumor lysis syndrome |
Investigations showed leucocytosis with monocytosis, thrombocytopenia, and massive hepatosplenomegaly. The child met the criteria for JMML on the investigations (Splenomegaly, 8%-blasts on Peripheral smear, leucocytosis, absolute monocytosis, myeloid precursors |
|
Sequencing result |
GATA1, SETBP1 |
PTPN11, SETBP1 |
PTPN11, SETBP1 |
|
Details of variants |
1. Delins variant (chrX:g.48791258_48791271delinsTCAGGCAGT; c.149_162delinsTCAGGCAGT) in the GATA1 gene (561X, 8.4% -MAF). 2. Frameshift deletion (chrX:g.48791243_48791246del; c.134_137del) 560X, 24.8%-MAF in GATA1 gene ([Fig. 1]) 3. SETBP1 gene mutation at c.2843G > A (ENST00000649279.2) in Exon 4 was found (859X, MAF- 48.9%) ([Fig. 1]) |
1. PTPN11 mutation {(chr12:g.112450406G > A; c.226G > A) not reported in ExAC and 1000 genomes databases[VCV000013336.5]} 2. SETBP1 mutation [(chr18:g.44951942G > A; c.2602G > A) gain-of-function mutation MAF-0.002%, not reported in 1000 genomes database] (Fig. 2A and B) |
1. PTPN11 gene [c.179G > T (ENST00000635625.1) 51X, MAF- 49.3%]. 2. SETBP1mutation [c.1102A > G (ENST00000649279.2) with heterozygous allele burden (50%) and 258X depth read (Fig. 3A and B) |
|
Treatment |
Supportive |
Supportive |
On supportive transfusion care, awaiting BMT |
|
Outcome |
Mortality at day 2 of admission |
Mortality at day 4 of admission |
Alive and hemodynamically stable on transfusion |
|
Remarks |
Never reported in literature co-association of SETBP1 with GATA1in a patient could highlight the inter-related myeloid pathway in Leukemia and MPNS |
The child was detected to have concurrent. The rare presentation as tumor lysis syndrome could be attributable to co-existent PTPN-11 and SETBP1 mutation in the same clone by simultaneous or additional hit |
SETBP1 in JMML in our case three did not have a dismal outcome, suggesting the SETBP1 and PTPN11 could be heteroclonal or subclonal, rather than concomitant in single malignant clone |
| Fig 1 :The morphological features, flow-cytometry characters, and sequencing result of patient 1 in the left-side plane and the sequencing results for patients 2 (2A and B) and patient 3 (3A and B) on the right-side pane.
SETBP1 co-expression leads to increased phosphorylated-driven MAP kinases, with a possible role of trametinib in these disorders.[2] Co-occurrence with other known mutations highlights the interrelated roles of the downstream leukemoid pathogenesis in these strikingly different neoplasms. The case of TAM with SETBP1 could initiate new insight into myeloid leukemogenesis. Next-generation sequencing is essential in the diagnostic management of all pediatric myeloproliferative disorders and can improve outcomes with better risk stratification.
Conflict of Interest
None declared.
Acknowledgment
The sequencing panel for JMML and TAM for these patients supported by our NGO partner Cankids Kidscan, New Delhi, performed at a CAP-accredited laboratory.
Ethical Statement
All ethical consideration and permissions were obtained for preparation of manuscript.
* Joint first authors-Both contributed equally.
-
References
- Shiba N, Ohki K, Park MJ. et al. SETBP1 mutations in juvenile myelomonocytic leukaemia and myelodysplastic syndrome but not in paediatric acute myeloid leukaemia. Br J Haematol 2014; 164 (01) 156-159
- Carratt SA, Braun TP, Coblentz C. et al. Mutant SETBP1 enhances NRAS-driven MAPK pathway activation to promote aggressive leukemia. Leukemia 2021; 35 (12) 3594-3599
- Locatelli F, Niemeyer CM. How I treat juvenile myelomonocytic leukemia. Blood 2015; 125 (07) 1083-1090
- Piazza R, Magistroni V, Redaelli S. et al. SETBP1 induces transcription of a network of development genes by acting as an epigenetic hub. Nat Commun 2018; 9 (01) 2192
- Alfayez M, Issa GC, Patel KP. et al. The clinical impact of PTPN11 mutations in adults with acute myeloid leukemia. Leukemia 2021; 35 (03) 691-700
Address for correspondence
Publication History
Article published online:
09 June 2023
© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
| Fig 1 :The morphological features, flow-cytometry characters, and sequencing result of patient 1 in the left-side plane and the sequencing results for patients 2 (2A and B) and patient 3 (3A and B) on the right-side pane.
-
References
- Shiba N, Ohki K, Park MJ. et al. SETBP1 mutations in juvenile myelomonocytic leukaemia and myelodysplastic syndrome but not in paediatric acute myeloid leukaemia. Br J Haematol 2014; 164 (01) 156-159
- Carratt SA, Braun TP, Coblentz C. et al. Mutant SETBP1 enhances NRAS-driven MAPK pathway activation to promote aggressive leukemia. Leukemia 2021; 35 (12) 3594-3599
- Locatelli F, Niemeyer CM. How I treat juvenile myelomonocytic leukemia. Blood 2015; 125 (07) 1083-1090
- Piazza R, Magistroni V, Redaelli S. et al. SETBP1 induces transcription of a network of development genes by acting as an epigenetic hub. Nat Commun 2018; 9 (01) 2192
- Alfayez M, Issa GC, Patel KP. et al. The clinical impact of PTPN11 mutations in adults with acute myeloid leukemia. Leukemia 2021; 35 (03) 691-700