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Identical Dual H3 Phosphoacetylation in G0/G1 and G2/M Phase of Cell Cycle: Any Functional Relevance?
CC BY 4.0 · Indian J Med Paediatr Oncol 2024; 45(S 01): S1-S16
DOI: DOI: 10.1055/s-0044-1788241
*Corresponding author: (e-mail: sgupta@actrec.gov.in).
Abstract
Background: Aberration in histone posttranslational modifications is associated with different diseases. The crosstalk among histone marks has been shown to play an important role in gene regulation. Recently, we have shown that H3S10phK14ac is present in both G0/G1 and G2/M phases of the cell cycle; however, the functional relevance is unknown.
Material and Methods: The gastric cancer cells were enriched in the G0/G1 phase by sorting and M phase by nocodazole. Histones were isolated from synchronized cells and studied using western blotting. Cell-cycle-specific RNA was processed for sequencing. An in vivo sequential gastric carcinogenesis model using carcinogen methyl nitroso urea and salt is being established.
Results: Around 90% of cells were synchronized in the respective phases by respective methods. The cell cycle-specific RNA-seq analysis has shown pathways involved in transcription and signaling to be upregulated in G0/G1. In contrast, in the G2/M phase, pathways related to G2M transition and cell cycle are upregulated. The in vivo sequential gastric carcinogenesis model has shown histopathological changes such as hyperplasia, adenomatous polyp, and dysplasia. The immunohistochemistry with specific histone marks, H3S10ph, and H3K14ac have shown altered levels in the early stages of gastric tumor development.
Conclusion: The modification, H3S10phK14ac, is known to regulate the transcription of key genes such as c-fos, c-jun, and cytokines. However, its presence in both phases of the cell cycle needs to be defined for its functional relevance in two phases of the cell cycle.
Publication History
Article published online:
08 July 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
*Corresponding author: (e-mail: sgupta@actrec.gov.in).
Abstract
Background: Aberration in histone posttranslational modifications is associated with different diseases. The crosstalk among histone marks has been shown to play an important role in gene regulation. Recently, we have shown that H3S10phK14ac is present in both G0/G1 and G2/M phases of the cell cycle; however, the functional relevance is unknown.
Material and Methods: The gastric cancer cells were enriched in the G0/G1 phase by sorting and M phase by nocodazole. Histones were isolated from synchronized cells and studied using western blotting. Cell-cycle-specific RNA was processed for sequencing. An in vivo sequential gastric carcinogenesis model using carcinogen methyl nitroso urea and salt is being established.
Results: Around 90% of cells were synchronized in the respective phases by respective methods. The cell cycle-specific RNA-seq analysis has shown pathways involved in transcription and signaling to be upregulated in G0/G1. In contrast, in the G2/M phase, pathways related to G2M transition and cell cycle are upregulated. The in vivo sequential gastric carcinogenesis model has shown histopathological changes such as hyperplasia, adenomatous polyp, and dysplasia. The immunohistochemistry with specific histone marks, H3S10ph, and H3K14ac have shown altered levels in the early stages of gastric tumor development.
Conclusion: The modification, H3S10phK14ac, is known to regulate the transcription of key genes such as c-fos, c-jun, and cytokines. However, its presence in both phases of the cell cycle needs to be defined for its functional relevance in two phases of the cell cycle.
No conflict of interest has been declared by the author(s).
Publication History
Article published online:
08 July 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India