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Evaluation of Curcumin as a Protective Agent for Caenorhabditis elegans Exposed to High Glucose Diet
CC BY 4.0 · Indian J Med Paediatr Oncol 2024; 45(S 01): S1-S16
DOI: DOI: 10.1055/s-0044-1788217
*Corresponding author: (e-mail: mrunmayeerade2411@gmail.com).
Abstract
Background: Curcumin, derived from Curcuma longa, exhibits antioxidant, anti-inflammatory, and antidiabetic properties. It lowers glucose levels, enhances insulin production, and has been investigated for effects on lifespan and fat accumulation. This study focuses on understanding how curcumin may mitigate high-glucose diet-induced stress in Caenorhabditis elegans, a model for hyperglycemia research.
Materials and Methods: N2 Bristol WT C. elegans were subjected to high-glucose diet from L1-adult, followed by curcumin treatment. Thrashing and pharyngeal pumping assays assessed locomotor and feeding behavior. Nitric oxide assay gauged in-vivo oxidative stress, while the DPPH assay measured curcumin’s antioxidant potential and its ability to scavenge reactive oxygen species.
Results: Damage or stress to C. elegans was estimated by aberrant behavior. Deviance from normal behavior was proportional to glucose concentrations. Recovery was observed with curcumin treatment. There is increase in ROS with increasing glucose concentrations. When worms exposed to 400 mM of glucose are treated with 100 µM of curcumin, ROS levels decreased by 14%. Curcumin has the ability to scavenge 50% (percentage inhibition - IC50 value) of the free radicals at 40 µM/10 mL, indicating its effectiveness as an antioxidant.
Conclusion: High-glucose diet induces stress in C. elegans as observed by the aberrant behavioral patterns. This could be associated to generation of ROS molecules as indicated previously by literature. Curcumin being an antioxidant molecule could reduce ROS and therefore manage oxidative stress.
Publication History
Article published online:
08 July 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
*Corresponding author: (e-mail: mrunmayeerade2411@gmail.com).
Abstract
Background: Curcumin, derived from Curcuma longa, exhibits antioxidant, anti-inflammatory, and antidiabetic properties. It lowers glucose levels, enhances insulin production, and has been investigated for effects on lifespan and fat accumulation. This study focuses on understanding how curcumin may mitigate high-glucose diet-induced stress in Caenorhabditis elegans, a model for hyperglycemia research.
Materials and Methods: N2 Bristol WT C. elegans were subjected to high-glucose diet from L1-adult, followed by curcumin treatment. Thrashing and pharyngeal pumping assays assessed locomotor and feeding behavior. Nitric oxide assay gauged in-vivo oxidative stress, while the DPPH assay measured curcumin’s antioxidant potential and its ability to scavenge reactive oxygen species.
Results: Damage or stress to C. elegans was estimated by aberrant behavior. Deviance from normal behavior was proportional to glucose concentrations. Recovery was observed with curcumin treatment. There is increase in ROS with increasing glucose concentrations. When worms exposed to 400 mM of glucose are treated with 100 µM of curcumin, ROS levels decreased by 14%. Curcumin has the ability to scavenge 50% (percentage inhibition - IC50 value) of the free radicals at 40 µM/10 mL, indicating its effectiveness as an antioxidant.
Conclusion: High-glucose diet induces stress in C. elegans as observed by the aberrant behavioral patterns. This could be associated to generation of ROS molecules as indicated previously by literature. Curcumin being an antioxidant molecule could reduce ROS and therefore manage oxidative stress.
No conflict of interest has been declared by the author(s).
Publication History
Article published online:
08 July 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India