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Chemoresistance Characterization Using Raman Spectroscopy: A Pilot Study
CC BY 4.0 · Indian J Med Paediatr Oncol 2024; 45(S 01): S1-S16
DOI: DOI: 10.1055/s-0044-1788234
*Corresponding author: (e-mail: sgupta@actrec.gov.in/mchilakapati@actrec.gov.in
Abstract
Background: Chemoresistance is a major hurdle in cancer therapy and it is necessary to understand and develop methods to predict chemoresistance to ensure better patient survival. Vibrational spectroscopy techniques such as Raman spectroscopy (RS) is widely explored as a tool to achieve this outcome.
Material and methods: We have used RS to characterize chemoresistant cell lines—HeLa, SiHa, HepG2, and Hep3B. Doxorubicin-resistant cell lines were developed from the parental counterparts. Raman spectra were recorded from the cell pellets and mean spectra were computed. The data were used to perform multivariate analyses PCA and PC-LDA.
Results: The Raman spectra revealed changes in important biomolecules—proteins, nucleic acids, cytochromes, and lipids (Table 1). The mean spectra of parental cell lines were different than the resistant cell types. The PC-LDA scatter plot in HeLa gave 82.53
*Corresponding author: (e-mail: sgupta@actrec.gov.in/mchilakapati@actrec.gov.in
Abstract
Background: Chemoresistance is a major hurdle in cancer therapy and it is necessary to understand and develop methods to predict chemoresistance to ensure better patient survival. Vibrational spectroscopy techniques such as Raman spectroscopy (RS) is widely explored as a tool to achieve this outcome.
Material and methods: We have used RS to characterize chemoresistant cell lines—HeLa, SiHa, HepG2, and Hep3B. Doxorubicin-resistant cell lines were developed from the parental counterparts. Raman spectra were recorded from the cell pellets and mean spectra were computed. The data were used to perform multivariate analyses PCA and PC-LDA.
Results: The Raman spectra revealed changes in important biomolecules—proteins, nucleic acids, cytochromes, and lipids (Table 1). The mean spectra of parental cell lines were different than the resistant cell types. The PC-LDA scatter plot in HeLa gave 82.53