PDF 
Views 
Share
Chemo-resistance Induced in Patient-Derived OSSC Cell Lines Leads to Hybrid EMT State and Enhanced Stemness
CC BY 4.0 · Indian J Med Paediatr Oncol 2024; 45(S 01): S1-S16
DOI: DOI: 10.1055/s-0044-1788231
*Corresponding author: (e-mail: swaghmare@actrec.gov.in).
Abstract
Background: Cancer stem cells (CSCs) within the tumor are a key factor responsible for acquisition of chemoresistance in patients that leads to tumor relapse. There are several studies that have shown their role in imparting chemo-resistance through signaling and multiple molecular mechanisms in various cancers.
Methods: We used the OSCC cell lines—ACOSC3 and ACOSC4. We performed the MTT assay to evaluate the IC50 values of Cisplatin, 5FU, and docetaxel for both cell lines. Subsequently, we characterized the chemo-resistant cell lines by performing in vitro experiments such as immunofluorescence assay, real-time PCR, and cell cycle analysis.
Results: After calculating the IC50 values, we started the drug treatment of the cell lines ACOSC3 and ACOSC4 with single and multiple drugs. Our results showed an increase in the colony-forming potential, as well as invasion and migration in the chemoresistant cells as compared to the parental cells. Further, in vitro assay showed increased orosphere formation in the chemoresistant cells as compared to the parental. In addition, cell cycle analysis of the chemoresistant versus parental population showed a difference in the S-and G2-M phases. The real-time PCR and immunofluorescence assays showed an increase in both E-cadherin and Vimentin in chemoresistant cells.
Conclusion: Our data showed the presence of a hybrid EMT state, increased invasion and migration potential, and increased colony forming potential in the chemoresistant cells as compared to the parental cells.
Publication History
Article published online:
08 July 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
*Corresponding author: (e-mail: swaghmare@actrec.gov.in).
Abstract
Background: Cancer stem cells (CSCs) within the tumor are a key factor responsible for acquisition of chemoresistance in patients that leads to tumor relapse. There are several studies that have shown their role in imparting chemo-resistance through signaling and multiple molecular mechanisms in various cancers.
Methods: We used the OSCC cell lines—ACOSC3 and ACOSC4. We performed the MTT assay to evaluate the IC50 values of Cisplatin, 5FU, and docetaxel for both cell lines. Subsequently, we characterized the chemo-resistant cell lines by performing in vitro experiments such as immunofluorescence assay, real-time PCR, and cell cycle analysis.
Results: After calculating the IC50 values, we started the drug treatment of the cell lines ACOSC3 and ACOSC4 with single and multiple drugs. Our results showed an increase in the colony-forming potential, as well as invasion and migration in the chemoresistant cells as compared to the parental cells. Further, in vitro assay showed increased orosphere formation in the chemoresistant cells as compared to the parental. In addition, cell cycle analysis of the chemoresistant versus parental population showed a difference in the S-and G2-M phases. The real-time PCR and immunofluorescence assays showed an increase in both E-cadherin and Vimentin in chemoresistant cells.
Conclusion: Our data showed the presence of a hybrid EMT state, increased invasion and migration potential, and increased colony forming potential in the chemoresistant cells as compared to the parental cells.
No conflict of interest has been declared by the author(s).
Publication History
Article published online:
08 July 2024
© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India