Cervical Angiomyolipoma Coexisting with Endometrial Carcinoma in the Absence of Tuberous Sclerosis: A Rare Case Report
CC BY-NC-ND 4.0 ? Indian J Med Paediatr Oncol 2021; 42(03): 305-308
DOI: DOI: 10.1055/s-0041-1733822
Abstract
Angiomyolipoma (AML) is a rare benign mesenchymal neoplasm composed of variable admixture of blood vessels, smooth muscle, and adipose tissue; most commonly located in kidney, and is usually associated with tuberous sclerosis. Extrarenal AML has been reported in various sites, although infrequently in female genital tract (FGT). AML in cervix is extremely rare and only six cases have been reported so far. A 46-year-old postmenopausal female presented with lower abdominal pain, bleeding for 4 days and a polypoidal mass protruding through the cervical os. Endometrial curettings were suggestive of endometrial adenocarcinoma?endometrioid type (World Health Organization [WHO]). The subsequent hysterectomy specimen confirmed the diagnosis and the histologic grade was International Federation of Obstetrics and Gynecology (FIGO) grade I and stage was pT1aN0 (American Joint Committee on Cancer [AJCC], 8th edition). Cervical polyp showed AML. CD34 and smooth muscle actin (SMA) showed diffuse positivity in blood vessels and smooth muscle cells, respectively. HMB-45 was negative. This is the first ever reported case of AML coexisting with endometrial carcinoma in the absence of tuberous sclerosis. CD34 is a good marker for delineating the blood vessels and SMA for both the smooth muscle and vascular components. S100 is generally not needed to prove the adipose tissue component. HMB-45 is not consistently expressed in AML of female genital tract.
Keywords
angiomyolipoma - cervix - endometrial carcinoma - tuberous sclerosis - HMB-45
Publication History
20 September 2021 (online)
A-12, Second Floor, Sector -2, NOIDA -201301, India
Abstract
Angiomyolipoma (AML) is a rare benign mesenchymal neoplasm composed of variable admixture of blood vessels, smooth muscle, and adipose tissue; most commonly located in kidney, and is usually associated with tuberous sclerosis. Extrarenal AML has been reported in various sites, although infrequently in female genital tract (FGT). AML in cervix is extremely rare and only six cases have been reported so far. A 46-year-old postmenopausal female presented with lower abdominal pain, bleeding for 4 days and a polypoidal mass protruding through the cervical os. Endometrial curettings were suggestive of endometrial adenocarcinoma?endometrioid type (World Health Organization [WHO]). The subsequent hysterectomy specimen confirmed the diagnosis and the histologic grade was International Federation of Obstetrics and Gynecology (FIGO) grade I and stage was pT1aN0 (American Joint Committee on Cancer [AJCC], 8th edition). Cervical polyp showed AML. CD34 and smooth muscle actin (SMA) showed diffuse positivity in blood vessels and smooth muscle cells, respectively. HMB-45 was negative. This is the first ever reported case of AML coexisting with endometrial carcinoma in the absence of tuberous sclerosis. CD34 is a good marker for delineating the blood vessels and SMA for both the smooth muscle and vascular components. S100 is generally not needed to prove the adipose tissue component. HMB-45 is not consistently expressed in AML of female genital tract.
Keywords
angiomyolipoma - cervix - endometrial carcinoma - tuberous sclerosis - HMB-45
Introduction
Angiomyolipoma (AML) is a rare tumor first reported in kidney by Morgan et al in 1951.[1] It is a benign mesenchymal neoplasm that is composed of variable admixture of blood vessels, smooth muscle, and adipose tissue.[2] [3] [4] [5] [6] Most common location of this tumor is kidney, in which case, it is associated with tuberous sclerosis.[2] [4] [5] [6] [7] [8] [9] [10] Extrarenal AML has been reported in liver, retroperitoneum, mediastinum, lung, nasal cavity, oral cavity, ear lobe, salivary glands, pharynx, heart, large intestine, bone, soft tissue, skin, thoracic spine, spermatic cord, penis, thigh, foot and infrequently in female genital tract.[2] [3] [4] [5] [6] [8] [11] In female genital tract, body of uterus is the most common site of AML and other sites are cervix, fallopian tube, ovary, broad ligament, parametrium, vaginal wall, and vulva[2] [3] [4] [5] [7] [8] [9] [10] [11] [12] [13] AML in cervix is extremely rare and only six cases have been reported so far ([Table 1]).[4] [13]
S. No. |
Author(s) |
Journal |
Year |
Parts involved |
HMB-45 status |
---|---|---|---|---|---|
1 |
McKeithen et al |
Obstet Gynecol |
1964 |
Uterine corpus |
Not done |
2 |
Jacobs DS et al |
Am J Clin. Pathol |
1965 |
3 cases in uterine corpus |
Not done |
3 |
Demopoulos et al |
Am J Clin Pathol |
1973 |
4 cases in Uterine corpus |
Not done |
4 |
Katz DA |
Am J Obstet Gynecol |
1984 |
Fallopian tube |
Negative |
5 |
Lo Re et al |
Appl Pathol |
1987 |
Uterine corpus |
Not done |
6 |
Peth SC et al |
Br J Obstet Gynaecol |
1988 |
Vaginal wall |
Not done |
7 |
Sieinski et al |
Int J Gynecol Pathol |
1989 |
1 case in uterine corpus and 2 cases in cervix |
Not done |
8 |
Chen KT et al |
Gynecol Oncol |
1990 |
Vaginal wall |
Not done |
9 |
Laffargue et al |
Gynecol Oncol |
1993 |
Uterine corpus |
Negative |
10 |
Shintaku et al |
Pathol Int |
1996 |
Uterine corpus |
Not done |
11 |
Huang et al |
Chinese Med J |
2000 |
Cervix |
Negative |
12 |
Chetty et al |
J Gynecol Surg |
2000 |
Uterine corpus |
Not done |
13 |
Yaeghashi et al |
Pathol Int |
2001 |
Uterine corpus |
Negative |
14 |
Anderson et al |
Int J of Gynec Pathol |
2002 |
Ovary |
Positive |
15 |
Cli AP et al |
Gynecol Oncol |
2004 |
Uterine corpus |
Positive (associated with Tuberous sclerosis) |
16 |
Dara? E |
J Reprod Med |
2004 |
Uterine corpus |
Negative |
17 |
Sharma NH et al |
The Inter J of Pathol |
2006 |
Ovary |
Positive |
18 |
Jungsuk An et al |
J of Women?s Med |
2010 |
Parametrium |
Negative |
19 |
Shakuntala PN et al. |
Ind J Surg Onco |
2012 |
Broad ligament |
Negative |
20 |
Yilmaz et al |
Eur J Gen Med |
2013 |
Uterine corpus |
Negative |
21 |
Totev TP et al |
J Biomed Clin Res |
2014 |
Uterine corpus |
Negative |
22 |
Garg M et al |
J Can Res Ther |
2015 |
Vulva |
Not done |
23 |
Sharma NH et al |
Int J Medi Upd |
2017 |
Cervix |
Negative |
24 |
Monteiro R et al |
Int J Res Med Sci |
2019 |
Cervix |
Negative |
25 |
Walke V et al |
JMSCR |
2019 |
Cervix |
Not done |
26 |
Dutta S et al |
Autops Case Rep |
2020 |
Broad ligament |
Negative |
27 |
Present case |
? |
2020 |
Cervix |
Negative |
AML belongs to the family of tumors called perivascular epithelioid cell tumors.[4] In general, AML is associated with tuberous sclerosis in 5 to 50% of cases.[3] [8] Most of the AML arising from female genital tract is not reported to be associated with tuberous sclerosis.[7] [10]
Clinical presentation of AML is variable and may be asymptomatic or presents with menorrhagia/menometrorrhagia/abdominal pain/pelvic mass.[2] [4] [5] [11] Ultrasonographic findings are also nonspecific.[4] [10] Per speculum, it appears as firm globular mass.[3] [4] Cut surface is yellowish white, soft in consistency.[4] [5] [11] Microscopically, it presents as unencapsulated, polypoidal tumor lined by stratified squamous epithelium.[3] It is composed of three components: the smooth muscle fascicles, thick walled hyalinized blood vessels, and mature adipose tissue.[2] [3] [4] [5] [6] [11] Verhoeff Van Gieson demonstrates the lack of elastic fibers in blood vessels in this tumor.[3]
We report the first case of AML of cervix that presented along with adenocarcinoma of endometrium in the absence of tuberous sclerosis.
Case Report
P3L3, a 46-year-old female, presented with lower abdominal pain and postmenopausal bleeding for the last 4 days. Patient has attained menopause before 7 years. She had history of menorrhagia with passage of clots for the past 4 days. She did not have any other medical illness. No features of tuberous sclerosis were identified. Per speculum examination revealed a firm to hard, 4 ? 4 cm polypoidal mass protruding through the cervical os. On ultrasonogram, there was a 4.2 ? 3.6 cm posterior fibroid with cervical polyp and the endometrial thickness was 8.6 mm. Fractional curettage was done along with cervical polypectomy. The endometrial curetting yielded copious material. Microscopically, the curettings showed tumor composed of atypical cells arranged in villoglandular, complex glandular, papillary configuration and in solid sheets with six to seven mitotic figures/10 high power field (hpf). Clear cell change was noted in many foci. Some of the endocervical glands also showed cellular atypia. All features suggested malignancy. The polypectomy specimen from the cervix measured 3.5 ? 2 ? 1.5 cm and external surface was firm and gray white. Cut surface was homogenous and tan white in color. On microscopic examination, the cervical polyp showed a benign tumor lined by cervical epithelium that had been partially eroded. The tumor had been composed of an admixture of smooth muscle fascicles, lobules of adipocytes, and numerous blood vessels with hyalinization in varied proportions ([Figs. 1B] and [C]). No atypia or mitosis was seen. The polypoidal tumor of cervix showed CD34 ([Fig. 2A]) and smooth muscle actin (SMA) ([Fig. 2B]) diffuse positivity in blood vessels, and smooth muscle cells, respectively. HMB-45 was completely negative ([Fig. 2C]). Based on these findings, the cervical tumor was diagnosed as AML.
Magnetic resonance imaging abdomen with contrast was done that showed enlarged bulky uterus, fairly defined, homogenous enhancing lesion with a broad base, measuring 3.8 ? 3 ? 2.6 cm projecting into the endometrial cavity abutting the posterior myometrium with suspicious endocervical invasion, suggesting the features of endometrial carcinoma. Hence, a staging laparotomy was done. Later total abdominal hysterectomy specimen was received with bilateral salpingo-oophorectomy, pelvic, paraaortic lymph nodes, and attached peritoneum. Externally, uterus appeared unremarkable. Cut surface showed dilated uterine cavity. Endometrium showed diffuse polypoidal, gray brown, friable growth measuring 2.3 ? 1 ? 0.5 cm ([Fig. 3A]) and grossly myometrial invasion was identified.
Microscopically, sections from the posterior aspect of the body of uterus and fundus showed a tumor arising from the endometrium, which is composed of atypical columnar cells arranged in solid sheets, complex glandular and trabecular patterns with two to four mitotic figures/10 hpf ([Fig. 3B?E]). Less than 50% of the myometrium was seen to be invaded. The anterior aspect of uterine corpus, bilateral parametria, fallopian tubes, and ovaries was free of tumor. There were 9 paraaortic nodes and 24 pelvic nodes that were free of tumor deposits. It was diagnosed as Endometrial adenocarcinoma?endometrioid type (World Health Organization [WHO]), histologic grade I (International Federation of Obstetrics and Gynecology [FIGO]) and pT1aN0 (American Joint Committee on Cancer [AJCC], 8th edition).
Left ovary showed a cortical inclusion cyst and left fallopian tube showed a paratubal cyst. The right adnexa were unremarkable.
Discussion
AML belongs to the family of PEComas.[4] [6] [14] [15] PEComas include clear cell sugar tumors, myomelanocytic tumors, lymphangioleiomyomatosis, and AML.[4] [5] [6]
AML is extremely rare in the female genital tract.[3] [5] [6] AML in female genital tract usually arises in women over 40 years of age.[6] It is not included in the WHO classification of tumors of the female genital tract.[3] [5] [6] [10] [13]
AML is generally associated with tuberous sclerosis.[3] [6] [8] Patients with tuberous sclerosis have increased risk of developing renal tumors and 5 to 80% risk of developing AML[3] [4] [5] [6] [10]
In tuberous sclerosis, hamartomas have been described in various organs such as adenoma sebaceum and angiofibromas in skin, AML in kidney, lymphangiomyomas in lung, sclerotic lesions in bone, tubers in brain, rhabdomyomas in heart, and phakomas in eyes.[6] Until their clonal neoplastic proliferation was proven, these tumors were considered as hamartomas.[2] [4] [8] No atypia/increased mitotic activity is seen in AML.[3] [6] [11] [13] It may rarely show invasion of surrounding organs.[3] [4]
The differential diagnoses of AML include lipoleiomyoma, degenerated myoma, vascular leiomyoma, benign lipomatous tumor, and angiolipoma.[2] [3] [4] [6] [7] [14] Lipoleiomyoma is composed of adipose tissue and smooth muscle cells, but lacks the thick-walled blood vessels.[3] [4] [14] The thick-walled and hyalinized blood vessels in AML are appreciated better with trichrome stain.[4] [11] Degenerated myoma is distinguished from AML by ultrasonographic findings, without shadowing and irregular margins.[3] [6] Benign lipomatous tumor comprises only adipose tissue and lacks the other two components.5?In AML, SMA is positive in the smooth muscle component and blood vessel wall; CD34 is positive in the endothelial cells; S100 in the mature adipose tissue component.[5] [12] In our case, similar immunohistochemistry findings were found. But S100 staining was not performed.
All PEComas and hamartomas in tuberous sclerosis express HMB-45.8?HMB-45 is positive in the nonvascular smooth muscle cells in AML. This feature has been reported in AML arising from kidney, lymph node, parametrium, colon, but not in most of the AML from the female genital tract.[2] [3] [4] [5] [10] [12] [13] [14] [16] Twenty percent of AML are negative for HMB-45.[3] [6] [14] [16] Except in liver, most of the extrarenal AML are negative for HMB-45. Hence, it is proposed that HMB-45 reactivity is more related to abnormal TSC genotype, rather than AML.[8]
AML of cervix is rare and this is the first ever reported case of AML coexisting with endometrial carcinoma in the absence of tuberous sclerosis. CD34 is a good marker for delineating the blood vessels and SMA for both the smooth muscle and vascular components. S100 is generally not needed to prove the adipose tissue component. HMB-45 is not consistently expressed in AML of female genital tract. It is useful in the diagnosis of AML. But it is certainly not the definitive marker for AML[3] [14] [16]
Conclusion
AML is a tumor that can be diagnosed with the morphological findings alone without applying immunohistochemistry and even in the absence of tuberous sclerosis. The recurrence risk associated with AML is negligible as compared with malignant PEComas that has increased risk of recurrence and metastasis in the gynecological and genitourinary tract. This tumor is resected surgically, without any radical intervention, unless it is associated with endometrial carcinoma as reported in this case. The routine follow-up strategy for AML is mere observation with periodic pelvic and systemic examination.
Conflict of Interest
None declared.
Declaration of Patient Consent
The authors certify that they have obtained all appropriate patient consent forms. In the form the patient has given her consent for images and other clinical information to be reported in the journal.
References
- 1?Morgan GS, Straumfjord JV, Hall EJ.?Angiomyolipoma of the kidney. J Urol 1951; 65 (04) 525-527
- 2?Garg M, Duhan A, Bindroo S, Kaur J, Mahajan NC.?Isolated angiomyolipoma of vulva: A case report of an uncommon tumor at an uncommon site. J Cancer Res Ther 2015; 11 (03) 645
- 3?Monteiro R, Sharma S, Gupta S, Choudhary I.?Extrarenal angiomyolipoma in uterine cervix: rare presentation in unusual site. Int J Res Med Sci 2020; 8: 367-370
- 4?Walke V, Gaikwad A, Ratnaparkhi S, Kowe B.?Angiomyolipoma of the cervix: a case report. JMSCR 2019; 7 (03) 371-374
- 5?Tovet TP, Mateva SA, Nikolova MR, Gorchev GA.?Uterine angiomyolipoma: a case report, differential diagnosis with PEComa and review of the literature. J Biomed Clin Res 2014; 7 (01) 47-52
- 6?Yilmaz M, Ingec M, Isaoglu U, Sipal S.?Angiomyolipoma of the uterus. Eur J Gen Med 2013; 10 (03) 178-180
- 7?Peh SC, Sivanesaratnam V.?Angiomyolipoma of the vagina?an uncommon tumour. Case report. Br J Obstet Gynaecol 1988; 95 (08) 820-823
- 8?Anderson AE, Yang X, Young RH.?Epithelioid angiomyolipoma of the ovary: a case report and literature review. Int J Gynecol Pathol 2002; 21 (01) 69-73
- 9?Chen KT.?Angiomyolipoma of the vagina. Gynecol Oncol 1990; 37 (02) 302-304
- 10?Cil AP, Haberal A, Hucumenoglu S, Kovalak EE, Gunes M.?Angiomyolipoma of the uterus associated with tuberous sclerosis: case report and review of the literature. Gynecol Oncol 2004; 94 (02) 593-596
- 11?Katz DA, Thom D, Bogard P, Dermer MS.?Angiomyolipoma of the fallopian tube. Am J Obstet Gynecol 1984; 148 (03) 341-343
- 12?Shakuntala PN, Shilpashree M, Geethanjali S, Sharma SK.?Acute abdomen as an unusual presentation of broad ligament angiomyolipoma. A case report and review of literature. Indian J Surg Oncol 2012; 3 (04) 276-278
- 13?Yaegashi H, Moriya T, Soeda S, Yonemoto Y, Nagura H, Sasano H.?Uterine angiomyolipoma: case report and review of the literature. Pathol Int 2001; 51 (11) 896-901
- 14?Aung T, Goto M, Nomoto M. et al?Uterine lipoleiomyoma: a histopathological review of 17 cases. Pathol Int 2004; 54 (10) 751-758
- 15?Conlon N, Soslow RA, Murali R.?Perivascular epithelioid tumours (PEComas) of the gynaecological tract. J Clin Pathol 2015; 68 (06) 418-426
- 16?An J, Kim HN, Cho HY, Chung DH, Kim NR.?Uterine vascular leiomyoma with a fat component mimicking angiomyolipoma: a case report. J Women?s Med 2010; 3 (03) 130-133
Address for correspondence
Publication History
20 September 2021 (online)
A-12, Second Floor, Sector -2, NOIDA -201301, India
References
- Morgan GS, Straumfjord JV, Hall EJ.?Angiomyolipoma of the kidney. J Urol 1951; 65 (04) 525-527
- Garg M, Duhan A, Bindroo S, Kaur J, Mahajan NC.?Isolated angiomyolipoma of vulva: A case report of an uncommon tumor at an uncommon site. J Cancer Res Ther 2015; 11 (03) 645
- Monteiro R, Sharma S, Gupta S, Choudhary I.?Extrarenal angiomyolipoma in uterine cervix: rare presentation in unusual site. Int J Res Med Sci 2020; 8: 367-370
- Walke V, Gaikwad A, Ratnaparkhi S, Kowe B.?Angiomyolipoma of the cervix: a case report. JMSCR 2019; 7 (03) 371-374
- Tovet TP, Mateva SA, Nikolova MR, Gorchev GA.?Uterine angiomyolipoma: a case report, differential diagnosis with PEComa and review of the literature. J Biomed Clin Res 2014; 7 (01) 47-52
- Yilmaz M, Ingec M, Isaoglu U, Sipal S.?Angiomyolipoma of the uterus. Eur J Gen Med 2013; 10 (03) 178-180
- Peh SC, Sivanesaratnam V.?Angiomyolipoma of the vagina?an uncommon tumour. Case report. Br J Obstet Gynaecol 1988; 95 (08) 820-823
- Anderson AE, Yang X, Young RH.?Epithelioid angiomyolipoma of the ovary: a case report and literature review. Int J Gynecol Pathol 2002; 21 (01) 69-73
- Chen KT.?Angiomyolipoma of the vagina. Gynecol Oncol 1990; 37 (02) 302-304
- Cil AP, Haberal A, Hucumenoglu S, Kovalak EE, Gunes M.?Angiomyolipoma of the uterus associated with tuberous sclerosis: case report and review of the literature. Gynecol Oncol 2004; 94 (02) 593-596
- Katz DA, Thom D, Bogard P, Dermer MS.?Angiomyolipoma of the fallopian tube. Am J Obstet Gynecol 1984; 148 (03) 341-343
- Shakuntala PN, Shilpashree M, Geethanjali S, Sharma SK.?Acute abdomen as an unusual presentation of broad ligament angiomyolipoma. A case report and review of literature. Indian J Surg Oncol 2012; 3 (04) 276-278
- Yaegashi H, Moriya T, Soeda S, Yonemoto Y, Nagura H, Sasano H.?Uterine angiomyolipoma: case report and review of the literature. Pathol Int 2001; 51 (11) 896-901
- Aung T, Goto M, Nomoto M. et al?Uterine lipoleiomyoma: a histopathological review of 17 cases. Pathol Int 2004; 54 (10) 751-758
- Conlon N, Soslow RA, Murali R.?Perivascular epithelioid tumours (PEComas) of the gynaecological tract. J Clin Pathol 2015; 68 (06) 418-426
- An J, Kim HN, Cho HY, Chung DH, Kim NR.?Uterine vascular leiomyoma with a fat component mimicking angiomyolipoma: a case report. J Women?s Med 2010; 3 (03) 130-133